Mapping DNA sequence to transcription factor binding energy in vivo

“Mapping DNA sequence to transcription factor binding energy in vivo” by Stephanie L Barnes, Nathan M Belliveau, William T Ireland, Justin B Kinney and Robert Phillips https://doi.org/10.1101/331124 In this article, Barnes and colleagues constructed a libraries of strains in which GFP expression is controlled by a transcription factor, and the transcription factor (TF) binding sites were randomly mutated. Libraries were sorted by GFP fluorescence and sequenced to determine the likelihood that a given binding site corresponds to a range of fluroescence. »